Viral Wiki

The Wiki is currently in beta. Contact us here if you’re interested in participating in our Wiki Project!

What we’re trying to do…

  • Provide an easy to use wiki
    • to allow researchers to contribute to the wiki and knowledge base
    • with a user-friendly interface and content editor
    • that gives version history and sends email updates of recent changes
    • check back soon for specific tutorials
  • Rally the research community to provide up to date gene annotation
    • by adding links to research reviews/papers
    • by updating/correcting GenBank genome information
    • by identifying genes that need correction/clarification
  • Provide an opportunity for collaboration and sharing opinions:
    • click the Discussion tab and post in comment section
    • find experts in the particular area you have questions about
    • visit our Forum to find a larger research community

Sub Wikis

Last edited by jwong on April 11, 2012 at 8:16 am
 

One Response to Viral Wiki

  1. lateef says:

    HI

    This is lateef .I am presently doing Resarch in taiwan on virus infecting fishes usually grouper . i am trying to produce recombinant protein, for this i have to clone gene first . i worked on MCP gene and Tyrosine Kinase gene. I get the band for MCP gene , but i did not get it in case of tyrosine kinase Gene. I tryied it at least 10 times by changing every pramater, but still i did not get the band. i use all the same constituents as in case of MCP gene & also with positive control. More than that i designed new primar also but still not working .

    Also i do not get any unspecific.

    My first P rimar sequence

    Forward Primar seq.

    5″———— 3″

    TCATA GTGTT TTATA TACCC CAAAG T.

    Reverse Primar.

    ATGTC TGCAT GGCGT GCAGT.

    New primar sequence.( i added restriction sites -SacI and Xho1)

    5″—————–3″

    Forward.

    CAGCT CTCAT AGTGT TTTAT ATACC CC

    Reverse.

    CTCGA GTCGT ATGTC TGCAT GGC.

    So plese let me know where is the problem . i wasted alredy lot of time with this exp.

    Also i have got the sequence of original sequence of the local virus(virus collected from infected fish in taiwan)for primar designing i have designed it as per the sequence available on ncbi (virus of japan)

    now i want to compare the two sequences . how can i compare them

    Thank You

    Regards.